描述
用于快速定量水中的嗜肺军团菌和军团菌
军团病是军团菌导致的感染,通常因吸入气雾导致。每年报告患者人数超过9000例,是水传播疾病最常见的病因之一。
军团菌能够定植于人工水系统和装水设备中,导致以下场所产生严重问题:- 冷却塔可产生各种细菌的行业;
- 受污染热自来水或沐浴水是军团菌潜在爆发源以及可能影响虚弱患者的医院和护理设施;
- 温泉和游泳池等常见气溶胶分散的公共建筑;
- 公共区域的喷泉。
颇尔GeneDisc技术为水系统网络管理的军团菌风险管理提供实时的整套解决方案。
GeneDisc系统的优点
快速- 尽管培养方法需要长达12天才出结果,但颇尔GeneDisc方法能够在最快2个小时内定量军团菌 。
易于使用 — 易于使用
模块化- 系统模块化可满足全方位需求:可同时分析多达88个样品。
用于水网管理的解决方案
降低停机成本 – 出结果时间缩短,从而能够监测反应,避免对水网和系统关闭的干预迟缓。
采取合适有效的消毒措施–使用GeneDisc系统绘制水系统网络,识别和靶向污染管路和供水点。
根据NF T 90-471和ISO/TS 12869 – NF验证认证方法依据两个标准。
根据NF T 90-471和ISO/TS 12869 – NF验证认证方法依据两个标准。– GeneDisc板军团菌 DUO可以使用相同的制剂组合检测和定量军团菌以及嗜肺军团菌,不会增加上机时间。
嗜肺军团菌 ID ID
细菌 | 革兰氏-带有鞭毛的细菌 |
患病率 | 无处不在的水生生物(从饮用水到冷却塔气溶胶) |
疾病 | 军团菌(庞蒂亚克热、军团病) |
报告的人体病例 | 6,296 (ECDC,2010) 3,346 (CDC, 2010) |
Related Clusters, Outbreaks | 130 (ECDC, 2010) 10次为景观用水(CDC,2007-2008) 12次为饮用水(CDC,2007-2008) |
规格
How the System Works
Technical Information
Total Turnaround Time | 2 hours for clear water samples |
3 hours for complex water samples | |
PCR Cycle Time | 1 hour |
Limit of Detection | 5 GU/PCR well |
Limit of Quantification | 25 GU/PCR well |
Quantification Range | 25 – 250,000 GU/PCR well |
PCR Efficiency (e) | 75 % < e < 125 % |
Optimal Recovery | > 50 % |
Specificity | Wide range of strains tested for inclusivity and exclusivity |
Legionella pneumophila Calibrator (external quantitative control) | Included in each GeneDisc Plate to validate accuracy of quantification |
Internal Positive Control Per Sample Analysis | Detects presence of inhibitors in DNA extract sample |
类型
用途
应用
附加信息
Further Reading
- Yaradou, D. F. et al., 'Integrated real-time PCR for detection and monitoring of
Legionella pneumophila in water systems', Appl. Environ. Microbiol. 73: 1452-62.
2007 - Dusserre, E. et al., 'A PCR-based method for monitoring Legionella pneumophila in
water samples detects viable but noncultivable Legionella that can recover their
cultivability', Appl. Environ. Microbiol. 74 (15): 4817-4824. 2008 - Alleron, L. et al., 'Long-term survival of Legionella pneumophila in the viable but
nonculturable state after monochloramine treatment', Curr Microbiol. 57(5): 497-502.
2008 - Mietzner, S. M. et al. 'A rapid method for the detection of the nosocomial waterborne
pathogens Legionella spp. and Pseudomonas aeruginosa by real-time quantitative
PCR - A comparison with standard culture'. Presented at ICAAC/IDSA Meeting.
Washington, D.C. 2008
订购信息
Part Number | Description | Samples/pack |
Equipment | ||
EGDCV3A | GeneDisc Cycler Base Unit | |
EGDSV3A | GeneDisc Cycler Sub Unit | - |
EGDUL1A230 (EU) EGDUL1A120 (US) |
GeneDisc Ultra-Lyser (for clear and complex water samples) |
- |
EGDUP1A | GeneDisc Ultra-Purifier (for complex water samples) |
- |
Consumables | ||
ALEGPNE205 | Legionella pneumophila Standard DNA (5 tubes) |
- |
PENVI1096 | Extraction Pack Environment 01 (for clear or complex water samples) |
96 |
PENVI3100 |
Extraction Pack Environment 03 (for clear water samples) |
100 |
GLEGPNE206006 GLEGPNE212006 |
GeneDisc Plate Legionella pneumophila | 30 66 |
GLEGSPP206006 | GeneDisc Plate Legionella spp. | 30 |
GLEGDUO206006 | GeneDisc Plate Legionella DUO | 30 |
GLEGOID206006 GLEGOID212006 |
GeneDisc Plate Legionella ID | 36 72 |
We also offer a full product range for pathogen detection in food and for spoilage organisms in beverages.
Quantitative tests for pathogens in water (E. coli, Enterococcus…) are also available.
For more information including part numbers please contact us.
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